The Byzantine Museum is part of Archbishop Makarios III Foundation and is located in the old town center of Nicosia, close to St. John Cathedral and the Folk Art Museum. Icons dating back to the 9-19th centuries make up the largest part of the exhibition. Visitors will also find a range of Byzantine artworks such as books, holy vessels, clothing and jewelry. The museum is also known for its collection of repatriated valuables.

The museum building also houses a library containing publications and manuscripts on Byzantine and Greek history and folklore, classical literature and other reference materials. Guided tours are available in Greek and English. Students receive a 50% discount off the price of admission.